Hsa_circ_0009910 Regulates Cisplatin Sensitivity of Ovarian Cancer Cells by Targeting miR-455-5p /PAX2
Hsa_circ_0009910 Regulates Cisplatin Sensitivity of Ovarian Cancer Cells by Targeting miR-455-5p /PAX2
Lei Jiang, Yi Huang, Runfeng Yang, Xiaohui Jiang* and Hao Yan
ABSTRACT
The objective of this study was to investigate whether Hsa_circ_0009910 regulates cisplatin sensitivity of ovarian cancer cells by regulating the expression of miR-455-5p /PAX2. The expressions of circ_0009910, miR-455-5p and PAX2 mRNA in HOSEPiCs and ovarian cancer cells SKOV3 and SKOV3/DDP were detected by qRT-PCR. SKOV3/DDP cells were grouped according to the transfection of different genes. The sensitivity of SKOV3/DDP cells to cisplatin was detected by CCK8 assay, and the apoptosis of SKOV3/DDP cells induced by cisplatin was detected by flow cytometry.Western blot was used to detect the expressions of apoptotic proteins Bax, Bcl-2, cleaved caspase and PAX2 in SKOV3/DDP cells, and the targeted relationship between circ_0009910, miR-455-5p and PAX2 was verified by double luciferase. Our results showed that circ_0009910 and PAX2 genes were significantly up-regulated in SKOV3/DDP cells, and miR-455-5p was significantly down-regulated in SKOV3/DDP cells. circ_0009910 could enhance cisplatin sensitivity of SKOV3/DDP cells. circ_0009910 targeted miR-455-5p, and knockdown of circ_0009910 up-regulated the expression of miR-455-5p in SKOV3/DDP cells. Overexpression of miR-455-5p can enhanced cisplatin sensitivity of SKOV3/DDP cells. miR-455-5p targeted PAX2 and overexpressed miR-455-5p down-regulated the expression of PAX2 gene in SKOV3/DDP cells. Inhibition of miR-455-5p or overexpression of PAX2 reversed the enhanced cisplatin sensitivity of knockdown circ_0009910 on SKOV3/DDP cells. It is concluded that circ_0009910 enhanced cisplatin sensitivity of ovarian cancer cells SKOV3/DDP by regulating miR-455-5p /PAX2 axis.
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