Evaluation of the Immune Effect of Oral Sustained-Release Vaccine pcDNA3-3-1E-PLGA Microsphere of Eimeria acervulina in Chicken
Evaluation of the Immune Effect of Oral Sustained-Release Vaccine pcDNA3-3-1E-PLGA Microsphere of Eimeria acervulina in Chicken
Liyun Chang1*, Yumei Cai1, Chenghui Li1 and Jianhua Qin2*
ABSTRACT
To evaluate the immune effect of oral sustained-release vaccine pcDNA3-3-1E-PLGA microspheres of Eimeria acervulina in chicken, seven day-old chickens were randomly divided into three groups, namely the DNA microsphere vaccine of pcDNA3-3-1E-PLGA microspheres of Eimeria acervulina oral immunization group, the naked plasmid DNA vaccine pcDNA3-3-1E of Eimeria acervulina oral immunization group and control group, with 30 chickens in each group. The chickens were immunized with a dose of 50μg/chicken, respectively, and strengthened immunity one week later. At 7th, 14th, 21st, 28th, 35th day post-injection, respectively, after extracting the total DNA from fresh tissues of immunized chickens, the 3-1E gene was amplified by PCR, the serum IgG antibody was detected by routine ELISA, peripheral blood lymphocyte proliferation response was evaluated by cell proliferation assay. The results showed that a target band of 583 bp was obtained by PCR in DNA vaccine PLGA microspheres group at 7th, 14th, 21st, 28th, 35th day post-injection. No 3-1E gene target band was found in the control group and the naked plasmid DNA vaccine group. It indicated that plasmid pcDNA3-1E existed in tissues of immunized chickens with DNA microsphere vaccine, and the plasmid pcDNA3-1E released from microspheres sustained at least 35days. The lymphocyte proliferation of DNA vaccine PLGA microspheres group significantly increased (P <0.05) and the peripheral blood IgG levels were significantly higher (P<0.05) than that of the naked plasmid group and the control group, but no difference was found between naked plasmid DNA vaccine group and the control group (P>0.05). The results of the present study indicated that the immune response was induced in the chickens after oral delivery of oral sustained-release vaccine pcDNA3-3-1E-PLGA microspheres of Eimeria acervulina.
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