Selenium and Malondialdehyde Levels in Placental Retention Cases in Friesian Holstein (FH) Cows
Selenium and Malondialdehyde Levels in Placental Retention Cases in Friesian Holstein (FH) Cows
Dwi Walid Retnawati1,2, Mohamad Agus Setiadi3*, Iman Supriatna3, Ligaya Ita Tumbelaka3
ABSTRACT
Long gestation and parturition are capable of triggering oxidative stress leading to placental retention (RP). The production of cows is often affected by RP, resulting in economic losses for the local community in dairy farm. Therefore, this research aimed to measure selenium (Se) and Malondialdehyde (MDA) levels as a candidate for RP parameters in dairy farm. The measurement started with preliminary research of plasma MDA levels in 20 cows comprising 5 RP and 15 non-placental retention (NRP). This was followed by the determination of Se and MDA levels in 46 cows consisting of 21 RP and 25 NRP. The samples were obtained from clinical conditions of dairy cows in the local community in Indonesia which have the recording of RP. The blood samples were taken by 4 groups at different times such as at 3 weeks and 1 week prepartus, 1 day and 3 weeks postpartus through the coccygeal vein. Subsequently, Se and MDA levels were tested with ICP-MS and spectrophotometer, respectively. The results showed that Se levels of RP cows in 3 weeks prepartus (48.45 ng/mL), decreased significantly in 1 week prepartus (34.74 ng/mL) and 1 day postpartus (33.39 ng/mL), followed by an increase at 3 weeks postpartus (34.04 ng/mL). Se levels of NRP cows during 3 weeks prepartus (60.96 ng/mL), decreased significantly at 1 week prepartus (38.84 ng/mL) and 1 day postpartus (37.09 ng/mL) but increased at 3 weeks postpartus (39.53 ng/mL). MDA level of preliminary research showed that RP cows 3 weeks prepartus (2.12 nmol/mL) significantly increased at 1 week prepartus (5.89 nmol/mL) and 1 day postpartus (7.13 nmol/mL). MDA of NRP cows in the period of 3 weeks prepartus (1.92 nmol/mL) increased from (3.04 nmol/mL) to 1 day postpartus (4.14 nmol/mL). Additionally, the evaluation of MDA levels in RP cows in 3 weeks prepartus (2.98 nmol/mL) increased significantly at 1 week prepartus (6.43 nmol/mL). MDA of NRP cows at 3 weeks prepartus (2.01 nmol/mL) also increased at 1 week prepartus (3.15 nmol/mL). Based on these analyses, MDA from blood samples at 1 week prepartus could be used as a proper parameter of RP in dairy farm.
Keywords | Malondialdehyde, Oxidative, Placental retention, Selenium
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November 2024
Vol. 12, Iss. 11, pp. 2062-2300
