Proteomic and Immunological Identification of Antigens from Ticks with Artificial Antiserum
Proteomic and Immunological Identification of Antigens from Ticks with Artificial Antiserum
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
ABSTRACT
We aimed to screen novel tick antigens using two-dimensional gel electrophoresis (2-DE) followed by western blot analysis (WB) with artificial antiserum. A total of 105 Haemaphysalis longicornis (H. longicornis) ticks were selected to prepare protein samples. Some of them were used for 2-DE, and the others were used to immunize mice to collect tick antiserum for WB. Then, 2-DE followed by WB and MALDI-TOF was carried out to screen and identify tick antigens. We identified 19 protein spots representing 12 ORFs: 4 from ticks and 8 from cattle. Among the the 4 ORFs, Tropomyosin, Elongation factor 1-alpha (EF-1α) and Protein Disulfide Isomerase-2 (PDI-2) have been reported as antigens and even vaccine candidate proteins in ticks or other pathogens but not in H. longicornis. Few reports have described dual specificity phosphatase 3 (DUSP-3) in parasites as an antigen. We successfully screened four potential antigens and found that DUSP-3 is a potential novel antigen for ticks.
To share on other social networks, click on any share button. What are these?
October 2020
Vol. 52, Iss. 5, Pages 1631-2026
