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Nonpolio Enterovirus-C (NPEV-C) Strains Circulating in South- Western Nigeria and their Contribution to the Emergence of Recombinant cVDPV2 Lineages

Nonpolio Enterovirus-C (NPEV-C) Strains Circulating in South- Western Nigeria and their Contribution to the Emergence of Recombinant cVDPV2 Lineages

Temitope Oluwasegun Cephas Faleye1, Johnson Adekunle Adeniji1, 2 *

1Department of Virology, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria; 2WHO Na- tional Polio Laboratory, University of Ibadan, Ibadan, Oyo State, Nigeria.

Email: adek1808@yahoo.com

ABSTRACT

Recently, we showed that the nonstructural region of some of the cVDPV2 lineages described in Nigeria between 2005 and 2011 originated from NPEV-Cs circulating in Northern Nigeria. Here, we further investigate whether NPEV-Cs circulating in south-western Nigeria (SWN) contributed to the emergence of these lineages. In 2013 sewage contaminated water samples were collected from two sites in Lagos, Nigeria. Samples were concentrated and inoculated into MCF-7 and RD cell lines. Isolates from MCF-7 were passaged in RD and L20B cell lines while isolates from RD cell line were passaged in L20B cell line. Subsequently, all isolates were subjected to panenterovirus 5l-UTR, partial VP1 and EV-C-3Dpol/3’-UTR PCR assays, amplicons sequenced and subjected to phylogenetic analysis. Both sites yielded two isolates each on MCF-7 cell line while on RD cell line one site yielded one and the other seven isolates. None of the twelve isolates replicated on L20B cell line. All were positive for both the panenterovirus 5l-UTR and partial VP1 PCR reactions while only the four isolates recovered on MCF-7 where positive for the EV-C-3Dpol/3’-UTR-PCR assay. The eleven isolates with exploitable VP1 sequence data were identified as CVA13 (4 isolates), E3 (1 isolate), E7 (3 isolates) and E19 (3 isolates). Recombination analysis showed evidence of recombination events. Results of this study showed that the NPEV- Cs circulating in south-western Nigeria also contributed to the emergence of cVDPV2 previously described between 2005 and 2011. Hence, the risk of recombination between OPV and NPEV-C members to generate recombinant VDPVs in the region exist.

 

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Hosts and Viruses

1

Vol. 8, Iss. 1, Pages 1-22

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