Cytopathogenicity of Local Field Strains of Bovine Viral Diarrhea Virus Isolated from Apparently Health Buffaloes in Egypt
Cytopathogenicity of Local Field Strains of Bovine Viral Diarrhea Virus Isolated from Apparently Health Buffaloes in Egypt
H. A. Hussein1, N.A. Mohamed2, F.M. Mohamed2 and M.A. Shalaby1
ABSTRACT
The growth behavior of BVDV field strains isolated from apparently health buffaloes in cell culture was studied. The isolated viruses were classified into 3 groups based on the onset and severity of cytopathic effect (CPE). The cytopathogenicity of the isolates were different when these viruses were inoculated on BVDV-free and-infected cell lines. To study the discrepancies on the growth behavior of the local isolates, the viruses subjected to further passages on MDBK cells infected with non-cytopathic (NCP) strain of BVDV which was homologues (pair) to their genotype (type I). After four passages on cell culture. The local isolates demonstrated enhancement in their CPE and virus replication as determined by virus titration assay. Propagation of these viruses in BVDV-free cells for five passages showed decrease in the severity of CPE with marked difference in virus titer when compared with those passaged in BVDV infected cells. The present study. confirms that the cytopathogenicity of the local isolates of BVDV are directly related to the presence of a helper virus which could be NCP homologous or heterologous. After 12 passages in cell culture. the obtained BVDVs in the harvests of CP BVDV infected cells may represent naturally defecting interfering viruses as a result of recombination mechanism.
To share on other social networks, click on any share button. What are these?
