The occurrence of Sugarcane bacilliform virus on sugarcane in Egypt
The occurrence of Sugarcane bacilliform virus on sugarcane in Egypt
Aly M. Abdel-Salam
ABSTRACT
Sugarcane bacilliform virus (SCBV), Badnavirus, Caulimoviridae was
detected in the noble sugarcane (Saccharum officinarum) plants in Giza and Assiut
governorates showing leaf symptoms of pronounced flecks or freckles and vein
banding which turn later to chlorotic and necrotic strips. Polymerase chain reaction
(PCR) utilizing degenerate primers for badnaviruses for the reverse trancriptase,
RT/RNase genome regions of ORF III detected the virus in infected sugarcane leaves
and in its vector Saccharicoccus sacchari and in another unidentified
Pseudococcidae- mealybug. Amplicons for the RT/RNaseH motifs of the two virus
isolates were cloned, sequenced, and submitted to the GenBanak. Pair-wise nucleotide
identity indicated 86% identity between the Giza and Assiut isolates. NCBI-blastn
analysis for the two isolates with four SCBV accessions retrieved from the GenBank
indicated identity range of 66-91%. Phylogenetic analysis based on amino acid
sequence identity indicated the clustering of the two SCBV in a separate branch from
the other known SCBV; indicating the special nature of the Egyptian SCBV isolates.
Close serologic relationship between SCBV and BSV from Egypt was detected. The
Egyptian antiserum of BSV captured SCBV antigens in leaves and insect vectors in
Immunocapture (IC) PCR analysis. The present study represents the first record of
SCBV presence in Egypt.
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July 2014
Vol. 11, Iss. 2, Pages 1-310
