1Beijing Institute of Animal and Veterinary Science, Chinese Academy of Agricultural Science, Beijing 100193, China
2Harbin Sports University, Harbin, 150008, China
Tengfei Luand Wenhua Peicontributed equally to this article.
* Corresponding author: wjguan86@iascaas.net.cn
ABSTRACT
With the self-renewal capacity and the potentiality of differentiating into various cells, stem cell is a prospective source for tissue engineering. Amniotic mesenchymal stem cells (AMSCs) obtained from amnion do not evoke ethical issues, as they do with the embryonic stem cells. However, a good many experimental tissues and organs were obtained from model animals but rarely from livestock. In this study, AMSCs were obtained from aborted sheep fetuses (1-3 month) under sterile conditions. Primary AMSCs were sub-cultured to passage 25 in vitro. The gene of Oct-4, Rex-1, CD29, CD44, CD71, and CD73 were identified by RT-PCR and immunofluorescence technique. The results showed that they were positive in AMSCs. The growth of different passages cells typically appeared in S curve. Furthermore, AMSCs successfully differentiated into osteoblasts, adipocytes, and hepatocyte-like cells after procedure induction. The results showed that the AMSCs obtained from sheep demonstrated the features of multipotent stem cells. Therefore, AMSCs could be a good option for transplantation for treatment and regenerative medical projects.
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