Phylogenetic Analysis of VP2 Gene in Canine Parvovirus Isolates in India and their Molecular Implications
Phylogenetic Analysis of VP2 Gene in Canine Parvovirus Isolates in India and their Molecular Implications
Pavana Jyothi Vanjavaka1, Mouradam Veerasami2, Mohana Subramanian Bhaskaran2*, Vijay A.K.B. Gundi1*
ABSTRACT
Canine parvovirus (CPV) is a contagious and pathogenic virus in puppies and dogs. It was first reported in 1978; however, it was later replaced by three antigenic variants at different periodic intervals that now circulate globally with random strains. This particular study aimed to determine the genetic changes in the VP2 gene, which are responsible for creating different antigenic variants, and to understand their impact on pathogenicity. In contrast, molecular characterization has become an essential tool in comprehending the evolution of viruses. For studying the molecular level changes, 28 field isolates were collected from various regions in India and subjected to PCR for amplification, with sequencing of a partial region of the VP2 gene. Among these isolates, 27 samples were positive for CPV, of which one was CPV 2b while the remaining were CPV 2a, and one isolate was CPV negative. The obtained sequences were submitted to NCBI to get gene accession numbers, and the phylogenetic tree was created to deduce the relationship between the different isolates of dogs, with a distance matrix derived from sequence variations in the genomes.
Keywords | Canine parvovirus, Hemorrhagic enteritis, VP2 gene, Maternal antibodies, CPV variants, Immunisation
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July 2024
Vol. 12, Iss. 7, pp. 1206-1409
