Virulence Potential of Aspergillus flavus Isolated from Dogs
Virulence Potential of Aspergillus flavus Isolated from Dogs
Salma Ali Munshed*, Fadwa Abdul Razaq Jameel
ABSTRACT
Aspergillus flavus is the cause of invasive and non-invasive Aspergillosis. The secretion of hydrolytic enzymes is considered a virulence factor in this species. The question of whether Aspergillus species can actually create biofilms has gained attention in recent times. Our work aimed to isolation and identification of Aspergillus flavus from dogs with study and evaluate some of virulence factors of this fungus like proteinase, hemolytic activity and biofilm formation in Laboratory. Fifty clinical isolates of respiratory infections in dogs were collected from several locations in Baghdad from Al Rusafa district (Zayouna, Al-Mashtel Street, Adamiyah; and Al-Karkh district (Baghdad Veterinary Hospital) during November 2023 to January 2024. The macroscopically and microscopically examination of this fungus was used to diagnosis of fungal elements. After that, detection of virulence factors for Aspergillus flavus was done using special media for hydrolysis albumin, hemolysis action and microplate technique for biofilm formation. This study showed a high percentage of Aspergillus spp.; at 42%, which included (Aspergillus flavus, Aspergillus niger at 18% respectively and Aspergillus terreus at 6%), The second fungus which isolated was penicillium spp. at 24% which also appeared an opportunistic filamentous fungal infections in dogs, with other fungi isolated at 34%. All isolates of Aspergillus flavus investigated highly significant differences in the hydrolysis of proteinase, hemolysis, and biofilm formation. Aspergillus flavus’s biofilm development showed the highest degree of virulence; these findings suggest that the biofilm phenotype is a more significant concept in mycological research.
Keywords | Aspergillus flavus, Dogs, Proteinase, Biofilm, Hemolytic activity
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November 2024
Special Issue 1, 2024
