Insights into Curcumin-Selenium Nanoparticles in Modulating Hormonal Levels and Promoting PTEN Gene Expression in Doxorubicin Treated Rats
Insights into Curcumin-Selenium Nanoparticles in Modulating Hormonal Levels and Promoting PTEN Gene Expression in Doxorubicin Treated Rats
Rawya Sh. Mohammed*, Baraa N. Al-Okaily
ABSTRACT
This study aimed to investigate the protective impact of synthesized curcumin selenium nanoparticles (CurSeNPs), using a hydroalcoholic extract of Curcuma longa rhizomes, in reducing disruption to hormonal profiles, redox homeostasis, inflammatory parameters, and ovarian tissue PTEN gene expressions in rats treated with doxorubicin. CurSeNPs were synthesized using sodium hydrogen selenite and a hydroalcoholic extract derived from Curcuma longa. The in vivo study utilized thirty-two mature female rats divided into four experimental groups over a two-week treatment period as follows: Rats in the control group (C) received distilled water orally, Group G1 received intraperitoneal injections of doxorubicin (4.40 mg/kg B.W), Group G2 was administered CurSeNPs (10.47 μg/kg B.W), and Group G3 received both doxorubicin and CurSeNPs at the same doses. Blood samples were collected from anesthetized rats; serum was analyzed to assess levels of FSH, estrogen, TGF-β, and NF-κB. Additionally, ovarian tissue was extracted to measure levels of malondialdehyde (MDA) and superoxide dismutase (SOD) concentrations, and to estimate ovarian gene expression of PTEN. The findings indicated a notable decrease in tissue SOD, serum FSH, and estrogen levels, along with an elevation in tissue MDA, serum TGF-β, NF-κB, and PTEN gene expression levels in the G1 group. However, the results demonstrated that administration of CurSeNPs in the G2 and G3 groups effectively improved oxidative stress-related factors, hormonal profiles, inflammatory criteria, and reduced expression of the PTEN gene. The present investigation has shown that CurSeNPs exhibit preventive actions against doxorubicin toxicity in adult female rats, possibly through their antioxidant, anti-inflammatory, and anticancer properties.
To share on other social networks, click on any share button. What are these?
