In Vivo Anti-Trypanosomal Activity of Basil Extract on Trypanosoma evansi
In Vivo Anti-Trypanosomal Activity of Basil Extract on Trypanosoma evansi
Esam A Razin1, Hassan Sobhy2, Tarek R. AboElnaga1, Asmaa A. Darwish1*, Rasha S. Mohammed1
ABSTRACT
Basil is a multifunctional medicinal plant. It confirmed its anti-trypanosomal activity in vitro before. This research aimed to study its effect against T. evansi in vivo. For this purpose, 28 parasite-free female rats were used, seven non-infected (control group (CG)), while the others were intraperitoneally injected with T. evansi and then equally divided into Trypanosoma Group (TG): which remained without treatment. Diminazene aceturate group (DAG): injected with Diminazene aceturate (3.5mg/kg) at 0, 14th, and 28th days. Basil group (BG): treated with essential oil of basil (850μL/kg) at 0, 14th, and 28th days. Blood samples were collected daily for monitoring the parasitemia and the clinicopathological parameters were measured at 0 and 49 days. Tissue sections were obtained from the liver, kidney, heart, lung, spleen, and brain on the 49th day, stained, and histopathologically examined. Although, both treatments presented a significant improvement (P<0.05) in their parasitological and clinicopathological results, DAG suffered from lower levels of GR than BG, and its organs microscopical examination clarified severe lesions similar to TG. While, BG clinicopathological parameters and histopathological results were so close to CG. CK, CK-MB, LDH, and GR had high sensitivity and NPV values and moderated specificity, PPV, LR, and accuracy rate values. Conclusion: Basil plant extract is an efficient anti-trypanosomal drug. CK, CK-MB, LDH, and GR are moderate biomarkers for disease diagnosis and its treatment monitoring.
Keywords | Anti-trypanosomal activity, Basil extract, Clinicopathological alterations, Histopathological alterations, Diminazene aceturate
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March 2023
Vol. 11, Iss. 3, Pages 355-516
