Sequence Analysis of N Gene of Peste Des Petits Ruminants Virus from Hail, Saudi Arabia
Sequence Analysis of N Gene of Peste Des Petits Ruminants Virus from Hail, Saudi Arabia
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
ABSTRACT
Peste des petits ruminants (PPR) is an endemic disease in Hail, Saudi Arabia where outbreaks are regularly reported. This study was carried out to analyze nucleoprotein (N) gene of PPRV from fatal infections in small ruminants. For this purpose, RNA was extracted from suspected animal samples (n=18) and were screened by real time-PCR. Additionally, to assess the phylogenomics, the N gene was amplified and sequenced from sheep and goats (n=12). Nucleotide identity among Hail strains was identified to be 96.2-100%, while identity with previously sequenced Saudi Arabian strains and with reference PPRV retrieved from GenBank was found to be 94.1-98% and 81.2-100%, respectively. Phylogenetic analysis revealed that Hail PPRV strains clustered in four separate groups within lineage IV indicating genetic divergence based on N gene. These findings further support the continuous characterization of PPRV strains in small ruminants to support the global eradication plan in 2030.
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