Molecular Identification of Honey Bee Viruses in some Egyptian Governments during 2021-2022
Molecular Identification of Honey Bee Viruses in some Egyptian Governments during 2021-2022
Dalia M.A. Elmasry1*, Dalia M. El-Husseini1, Asmaa A. Eissa1, Zakaria R. Elkanawati2, Momtaz A. Shahein3, Amany Adel4
ABSTRACT
Numerous illnesses, including bacteria, viruses, and protozoa, endanger the health and survival of honeybee colonies. Viruses, among ailments, pose a significant danger to the health and well-being of honeybees, causing beekeepers to be concerned about economic losses. One hundred samples has been collected from bee colony farms had bees loss 20% or more during 2021-2022 from some Egyptian governorates. The samples have been prepared and suspected viruses detection for Sacbrood virus (SBV), Israeli acute paralysis virus (IAPV), Acute bee paralysis virus (ABPV), Lake Sinai Viruses (LSV) ,Black queen cell virus (BQCV), Kashmir bee virus (KBV), Deformed wing virus (DWV) and Chronic bee paralysis virus (CBPV) by Reverse transcription polymerase chain reaction (RT-PCR). Then the positive samples have been genetically characterized by partial sequencing of respective genes of each virus. The results illustrated the domination of the DWV-B and LSV-4 viruses’ distribution in Egypt during all seasons with high incidence up to 88% and 82%, respectively. However, the other viruses have been detected with different incidence rate of BQCV, IAPV, SBV were 15%, 23%, 22%, respectively. KBV and CBPV were low incidence 4% and 1% without any spatial correlation. The obtained results for genetic diversity and relationships of bee viruses that are circulating in Egypt compared to earlier studies, show notable similarities and differences that provide insight into the genetic evolution and temporal dynamics of these viruses. As conclusion, the identification of mixed infections highlights the intricacy of viral interactions in honey bee colonies and highlights the necessity of more investigation into the processes responsible for co-infections.
Keywords: Egypt, SBV, IAPV, ABPV, LSV, BQCV, KBV, DWV, CBPV
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October 2024
Vol. 12, Sp. Iss. 1
