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Effect of Chronic Adrenocorticotropine Hormone Treatment on LPS-induced Slight Inflammation via Inhibiting Nuclear Translocation of Glucocorticoid-glucocorticoid Receptor Complex in Lung of Pig

Effect of Chronic Adrenocorticotropine Hormone Treatment on LPS-induced Slight Inflammation via Inhibiting Nuclear Translocation of Glucocorticoid-glucocorticoid Receptor Complex in Lung of Pig

Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1

1Department of Animal Husbandry and Veterinary Medicine, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400, P. R. China
2Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, P. R. China
3Key Laboratory of Animal Physiology & Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, P. R. China
 
Xuhui Zhang and Zhiyuan Sun contributed equally to this work and are considered as co-first authors.

*  Corresponding author: huihui19820131@163.com

ABSTRACT

The crosstalk between glucocorticoid (GC) and immune response is critical for the maintenance of homeostasis. However, it remains elusive whether chronic stress affects lipopolysaccharide (LPS) -induced inflammation via the crosstalk. Pigs were administered LPS injection with or without continuous adrenocorticotropine hormone (ACTH) pretreatment. Twenty-four 30-day-old Duroc × Landrace × Large White crossbred piglets (12 ± 0.5 kg) were randomly assigned to 4 treatments: control, LPS, ACTH, and ACTH+LPS groups. Each group consisted of 6 male piglets. All ACTH pigs and ACTH+LPS pigs were injected intramuscularly with ACTH (2.25 IU/kg body weight) for 7 consecutive injections at 6-h intervals. Then, LPS pigs and ACTH+LPS pigs were injected intramuscularly with LPS (15 μg/kg body weight). LPS upregulated levels of pulmonary IL-1β, IL-10, TLR2 gene mRNAs (P <0.01, P =0.006, P =0.04, respectively), and T-NOS, iNOS and cNOS proteins (P =0.002, P =0.028 and P =0.003, respectively) and increased serum tumor necrosis factor alpha (TNF-α) concentration (P <0.01), but ACTH treatment has no significant effect on them. Neither ACTH nor LPS treatment had any significant effect on levels of pulmonary SOD, CAT and MDA secretion, as well as expression of IL-6, TNF-α, IL-10, COX-2 and TLR4 mRNAs (P >0.05). Pulmonary cortisol level was inhibited by LPS (P =0.034), with a similar trend of inhibition by ACTH (P =0.062). Pretreatment with ACTH tended to downregulate total GR protein levels (P =0.084), and LPS tended to inhibit GR protein expression in the nucleus (P =0.088). Pulmonary expression level of Let-7i targeted TLR4 was significantly increased by ACTH treatment (P =0.013), but not with LPS. These results suggest that LPS may increase the expression of pulmonary TLR2 mRNA by inhibiting nuclear translocation of GC-GR, thus causing a slight inflammatory response in the lungs of pigs, which is not affected by chronic ACTH treatment. 
 

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Pakistan Journal of Zoology

February

Vol. 54, Iss. 1, Pages 1-501

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