Isolation and Identification of the Causal Virus Aucuba Disease in Egypt
Isolation and Identification of the Causal Virus Aucuba Disease in Egypt
A. S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik2; Nashwa M. A. Abd El Mohsen1; and H. M. Abdelmaksoud1
ABSTRACT
The infected potato plants showed aucuba symptom characteristic to this virus (brilliant and extensive yellow mottle on lower and middle leaves reaches their tips, brown patches and sunken brown areas were frequently observed on the tubers surface) were used as a virus source. Isolation and identification of the virus was performed through studying biological, serological, and molecular characters. Electron microscopy helped for illustration Of Morphology of virus particles as well as alteration in cell organelles. The virus systematically infects most Of Solanaceae plants. but become diverse to different hosts as it induced local lesions and top necrosis on Capsicum annuum L. cvs. California Wonder & Godion as well as Lycopersicon esculentum Mill. cvs. Streen B and Casel Rock. Solanum tuherosum L cv. Cara. Nicotiana glutinosa L. Nicotiana benthamiana gave systemic infection only. On the other hand. systemic symptomless infection had occurred with N. tabacum L. cvs. White Burley and Xanthine. Whereas PAMV didn't infect Ch. umaranticolor Cost. Reyn and D. metel L. The virus was transmitted by aphids (M persicae) in a non-persistent manner only when the aphids fed firstly on PVY N source for 10 min. This indicated a kind of synergistic effect between the two viruses which controlled by role of PVYN CP and HC-Pro. The ratio of transmission increased by exceeding the acquisition feeding period. The virus isolate showed positive reaction only with polyclonal antibodies specific to Potato aucuba mosaic virus (PAMV). Electron microscopy showed spindle-shaped like inclusions as aggregated virus particles slightly flexuous in longitudinal and cross sections of PAMV infected cells: the Vitus induced different abnormalities in the structure of chloroplasts and mitochondria. The CP genes of PAMV were detected using RT-PCR with specific primers.
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