Histo-cytopathological Effects of Cucumber Mosaic Cucumovirus on Squash leaves
Histo-cytopathological Effects of Cucumber Mosaic Cucumovirus on Squash leaves
M. Osman1, Kh. El-Dougdoug2, E. T. Abd El-Salam3, R.M. Taha4 and R.M. El-Hamidl
ABSTRACT
An isolate of Cucumber mosaic cucumovirus (CMV) infected cucumber plants was detected by DAS-ELISA and confinned by inoculation of Chenopodium amaranticolor. CMV-isolate was propagated on squash plants var. Eskandarani. After 15 days. systemic symptoms appeared in the newly formed leaves in the form of vein banding, severe mosaic, yellowing, malformation, and leaves wilting. Histopathological studies of CMV-infected squash leaves showed changes such as compact mesophyll, disintegrated and less lignified metaxylem, slight upward blistering of leaf blade, closed stomata, short palisade and rounded spongy cells as well as vascular elements of the midrib formed in two patches. The upper patch was completely divided in two parts. Scanning electron microscopy showed alteration in epidermal cells elongated, deformed, and disconnect hairs. decrease of opened stomata, destroyed and forming giant cells. The midrib cells of infected leaves showing clear elongations as well as deformation. Transmission electron microscopy illustrated highly thickened with clear secondary deposition of cell wall. The cytoplasm showed many vacuoles, several rounded hallow center inclusions, and aggregates of virus particles. Chloroplasts showed weak differentiated thylakoid system. rounded osmophilic globules and large deformed starch grains. Mitochondria appeared deformed without cristae. The nucleus appeared deformed with clear accumulation of marginal nuclear material. Multimembrane body and endoplasmic reticulum fragments also appeared.
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