PROP-1 Gene a Selective Marker of Wool Production in Sheep Breeds of Balochistan Pakistan
PROP-1 Gene a Selective Marker of Wool Production in Sheep Breeds of Balochistan Pakistan
Chandni Wajid1, Abdul Hameed Baloch1, Ahmed Nawaz Khosa1*, Hubdar Ali Kaleri2, Nasrullah Bangulzai1, Sarfraz Ali Fazlani1, Haleema Sadia3, Saeeda Kalsoom4, Muhammad Bilawal Arain2, Wassem Ali Vistro2
ABSTRACT
PROP1 gene plays a vital role in growth and development of wool production in mammals. The genetic variations in PROP1 gene in the sheep breeds of Pakistan are unknown. This study was conducted to compare wool color and fleece weight and to analyze the PROP1 gene of Balochi, Bibrik, Harnai and Rakhshani sheep breeds of Balochistan. The wool was sheared, its color and fleece weight were observed. The result reveled that wool color and fleece weight were black and white with 1.91 kg, full white, white with black muzzles and limb extremities with 1.83 kg, white gray mix with 1.85 kg, white with black muzzles and limbs extremities with 1.77 kg, for Balochi, Bibrik, Harnai and Rakhshani sheep breeds respectively. For PROP1 gene analysis, the blood sample were collected from the selected sheep breeds and processed for DNA extraction. Primers were designed using Primer 3 software and PCR was performed. PCR products were sequenced and analyzed for observing genetic variation in PROP1 gene of these four sheep breeds of Balochistan. The result for PROP1 gene analysis showed 7 variations including 3 frame shift and 4-point mutations with c.63G>A, c.83G>A, c.94G>T, c.104C>G, c.237G>A, c.432T>C and c.450C>T were observed in all three exons of PROP1 gene of Balochi, Bibrik, Harnai and Rakhshani sheep breed of Balochistan. The phenotypic variation related to wool production traits including the fleece weight and wool color with diverse pigmentation and genetic variations observed in the coding region of the PROP1 gene. This study indicates that the PROP1 gene may be used as a selective marker for the future selection of the sheep breeds for different wool productive traits.
Keywords | Genotypic variation, Phenotypic variation, Sheep, PROP1 gene, Wool trait.
To share on other social networks, click on any share button. What are these?
