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Rapid Virological and Molecular diagnosis of Lumpy skin disease virus

Rapid Virological and Molecular diagnosis of Lumpy skin disease virus

Hanaa A. Ahmed1 and Eman M. AboHatab2

Genome Research Unit1. Virology Research Dept.2
Animal Health Research Institute, Dokki, Egypt

ABSTRACT

Lumpy skin disease is an infectious viral disease of cattle, which is an important serious skin disease of cattle in Africa. In present study, twenty one samples in the form of nodular lesion of skin were collected at different stages during the course of the disease from private farms in Ismailia and Fayoum governorates during April to June 2013. Different diagnostic tests used for Isolation of LSD virus from the nodular samples by inoculation on chorioallantoic membrane (CAM) of embryonated chicken eggs (ECE) SPF at 9-11 day and Madin Darby bovine kidney (MDBK) cell culture. Identification of virus by indirect immuno fluorescent antibody technique (IFA) which was considered a rapid and accurate method for LSD antigen detection. nodules were subjected directly to PCR and Real time PCR for rapid and specific detection of LSDV, sixteen out of the twenty one nodular samples were confirmed positive by molecular methods. Eleven out of twenty one nodular samples were positive by (IFAT) in CAM, which prove that PCR and Real Time PCR are much sensitive and rapid diagnostic tool of LSD reflecting their importance in controlling the rapid spread of disease in Egypt.

 

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Journal of Virological Sciences

July

Vol. 8, Pages 1-27

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