Bacteriological Identification and Molecular Detection of Klebsiella pneumoniae from Pneumonic Camels in Al-Muthanna Province
Bacteriological Identification and Molecular Detection of Klebsiella pneumoniae from Pneumonic Camels in Al-Muthanna Province
Hayder M. Watban1,2, Nabeel M.H. Al-Maaly2*
ABSTRACT
Camels are one of the important animals in Al-Muthanna province; they exhibit meat, milk and hides for human consumption. Camels are characterized by resistance to hard condition, but still susceptible to many diseases such as bacterial infections. Klebsiella pneumoniae is one of the bacterial diseases that infect camels and induce pneumonia. In this study, one hundred camels were examined and samples were collected from lungs of pneumonic camels slaughtered in Al-Muthanna abattoir. Swabs were taken from lungs of slaughter camels, and cultured on blood and MacConkey agars. The bacterial growth was subjected into gram stain and subculture to Klebsiella pneumoniae selective media. Subsequently isolated bacteria were subjected to molecular detection using conventional PCR and sequencing. The finding showed that Klebsiella pneumoniae were 31 out of 74(41.89%) from total 100 camels that slaughter in the abattoir. This study indicates that Klebsiella pneumoniae reported higher infection rate as it is the predominant bacteria isolated from pneumonic camels which slaughter in Al-Muthanna abattoir.
Keywords | Camels, Klebsiella pneumoniae, pneumonia, PCR, Al-Muthanna province, Bacterial growth
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November 2023
Vol. 11, Iss. 11, pp. 1757-1910
