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Responses of Bacteria from Urban and Desert Soil for Bioplastic Accumulation

Responses of Bacteria from Urban and Desert Soil for Bioplastic Accumulation

Humaira Jabeen and Nazia Jamil*

Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan

*Corresponding author: Nazia Jamil, nazia.mmg@pu.edu.pk 

 

ABSTRACT

Abstract | Bacteria retain the ability to produce certain compounds to survive in unfavorable conditions; bioplastics is one of them. The aim of this study was to evaluate various environments for bioplastic producing bacteria. Bacterial strains were isolated from the desert and urban soil samples. A total of 39 strains isolated from Cholistan near Bahawalpur and 24 strains were isolated from various areas of Lahore. Bacterial strains were screened for the production and accumulation of bio-plastic. Only three strains from urban soil sample were able to produce polyhydroxyalkanoates (PHA). Strain Acinetobacter seohaensis produced 40.6% bio-plastic when supplemented with coconut oil. This percentage is even higher than the glucose, which was 16.6 % after 24 h of incubation. The other competent strains i.e. Exiguobacterium indicum, Bacillus acidiceler were not able to utilize the coconut oil but produced 5 and 22% bio-plastic when supplemented with 2% glucose after 24 h of incubation, respectively. The percentage of the bio-plastic produced when grown in nutrient broth was very low. The Fourier-transform infrared spectroscopy (FTIR) analysis of the extracted bio-plastic indicated the presence of the C=O functional group specific for the presence of polyhydroxyalkanoates, the absorbance peaks were specifically at 1600 cm-1. Ribo-typing of all the six strains were carried out, strains identified as Exiguobacterium indicum, Bacillus acidiceler, Acinetobacter seohaensis, Serratia proteamaculans, Trabulsiella guamensis and Serratia grimesii and showed 98 to 100% similarity with sequences available in public domains.
 

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Punjab University Journal of Zoology

June

Vol. 36, Iss. 1, pp. 1-123

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