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COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES

 Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*

 *Institute of Agri-Biotechnology and Genetic Resources, National Agricultural Research Centre, Islamabad, Pakistan. ** Oilseed Research Programme, Crop Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan. Corresponding author: tehrim.sadia@gmail.com

ABSTRACT

 The grape (Vitis vinifera L.) is the most valuable and widely cultivated horticultural crop worldwide. In the present study, different hormonal combinations in regeneration media were studied to determine the best combination for in vitro morphogenesis of grapevine accessions viz., 4494(1) Kowar Sufaid, 4472(12) Harker Agoon and 4492(1) Kowar Naray. When levels of benzylaminopurine (BAP) were increased in the basal MS media for culturing the grape plantlets, there was a corresponding increase in the number of shoots and root mass. Additionally, the shoot proliferation response to increasing level of BAP was found genotype dependent. Naphthaleneacetic acid (NAA) also showed a positive effect on in vitro growth responses (shoot proliferation and root induction) when combined with BAP. The media containing NAA, BAP and kinetin accelerated the growth in terms of shoot mass in two accessions, 4494(1) Kowar Sufaid and 4472(12) Harker Agoon and again the culture response was genotype dependent. There was a difference in the growth responses of the three accessions studied. The best rooting response was -1 obtained on 75% MS media containing 0.7 mgl , NAA. 

 

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Pakistan Journal of Agricultural Research

June

Vol. 34, Iss. 2, Pages 254-493

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