The Upregulated Exosome Protein ET-1 Induces the Increased Level of Ang-II and Contributes to the Progression of Pre-Eclampsia
The Upregulated Exosome Protein ET-1 Induces the Increased Level of Ang-II and Contributes to the Progression of Pre-Eclampsia
Yun Zhang*
ABSTRACT
Pre-eclampsia (PE) is one of the leading complications of pregnancy that contributes to the increasing morbidity and mortality. However, the effects of exosomes secretion and expression of ET-1 and Ang-II on PE progression has not been fully investigated. The PE (n=100) and normal (n=100) women with singleton pregnancy and in the second or third trimester of pregnancy were included in the present study. The healthy group included healthy women (n=100) aged 28 to 40. The blood samples were collected, and exosomes were isolated using commercial kit. The exosomes were observed by transmission electron microscope. Western blotting was done to detect the protein expression. Transmission electron microscope analysis revealed that exosomes could be significantly observed in the PE group. The expression of surface markers that included CD63 and CD81 were dramatically upregulated in PE group. The protein level of ET-1 and Ang-II were significantly increased in PE group, and the upregulated ET-1 and Ang-II expression were highly correlated with PE progression. The present study demonstrated that the exosome protein ET-1 upregulated the expression of Ang-II and contributed to the progression of PE.
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