Antiserum Production and Control Measures of Pepper Mottle Potyvirus using Different Applications
Antiserum Production and Control Measures of Pepper Mottle Potyvirus using Different Applications
El.-Kadyl, M.A.S; Badr2, A.B.; zein3, Saiwa N. and Khalifa4, M.A.A.
ABSTRACT
Pepper mottle virus (PepMoV) was purified with a high degree of purity and sufficient quantities for its antiserum production. The absorption spectrum of the purified virus had a min at 245nm and a max at 260 nm. The A260/280, A280/260 and Amax/min ratios were 1.30, 0.76 and 1.29, respectively. The estimated yield of the purified virus was 3.35 mg/100 g leaf tissues. Electron micrographs of the purified virus preparation revealed the presence of filamentous flexuous virus particles about 730-745nm long. Antiserum obtained after the second bleeding was 1/1024. The optimum concentrations of IgG and IgG conjugate were 1.0g/ml and 1/1000, respectively.The antigen dilution end point was 1/800. Ribavirin had a higher inhibitory effect (80%) when sprayed at lower concentration (the minimum inhibiting concentration is 0.0001%) before one hr of inoculation. Salicylic acid and Parahydroxy benzoic acid has the same effect (80% reduction) at concentration of 0.01% and 0.1, respectively in the treatments inoculated one hr. after sprays. Six different chemical compounds applied as foliar sprays with two concentrations for each were used to study their effect on the percentage of virus infection in pepper treated plants: Actellic application gave superior results (46.7%) in decreasing the infection percentage followed by either Sumithion or K.Z. oil (55.0%), whereas Potassium Soap spray gave infection percentage of 60.0% followed by Bio-fly (61.7%). Moreover Supper Royal treatment gave the lower effect in the percentage of virus infected plants (63.3%). Although, actellic treatment produced a higher yield as well as a higher weight of fruit followed by Sumithion and K.Z. oil (57.7&19.48,
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