Isolation and Antigenic and Molecular Characterization of G10 of Group A Rotavirus in Camel
Isolation and Antigenic and Molecular Characterization of G10 of Group A Rotavirus in Camel
Eman, Abo Hatab*; Hussein, HA.; El-Sabagh IM. and Saber, MS.
ABSTRACT
We are here reporting the successful Isolation and characterization of GIO serotype of group A rotaviruses from fecal samples collected from camel farms suffering from diarrhea in Alexandria and Esmalia governorates. After preparation of fecal samples and inoculation on MA 104 cell line for five passages, eight isolates were successfully isolated with a clear and reproducible CPE on the inoculated cells. The isolates were identified antigenically using VP6 monoclonal antibodies (MAbs) based antigens capture ELISA that able to detect any group A rotavirus. The viral RNA was extracted from the tissue culture harvest of the propagated viruses and RT-PCR using primers specific for VP6 and VP7 of group A rotaviruses was employed and confirmed the molecular characterization Of the isolates viruses with the correct and expected bands. The RT-PCR specific band of VP7 gene of two selected isolates was eluted from the agarose gel and sequenced using VP7 specific primers sequence. The obtained sequence was analyzed using computer software (BLAST) which revealed that both isolates had Maximum identity to the GIO serotype of group A bovine rotaviruses ranging from 90-93%. This is the first report on the circulation of G 10 serotype of group A rotaviruses in camel.
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