Submit or Track your Manuscript LOG-IN

Effects of Epidermal Growth Factor on Glutamine and Glucose Absorption by IPEC-J2 Cells Challenged by Lipopolysaccharide using the Ussing Chamber System

Effects of Epidermal Growth Factor on Glutamine and Glucose Absorption by IPEC-J2 Cells Challenged by Lipopolysaccharide using the Ussing Chamber System

Xiaopeng Tang1,2* and Kangning Xiong1,2

1School of Karst Science, Guizhou Normal University, Guiyang, China
2State Key Laboratory Cultivation for Karst Mountain Ecology Environment of Guizhou Province, Guiyang, China

*      Corresponding author: tangxiaopeng110@126.com

ABSTRACT

The aim of this study was to investigate the effects of epidermal growth factor (EGF) on glutamine and glucose absorption of IPEC-J2 cells using the Ussing chamber system. Cells were treated with: (1) EGF (0 ng / mL) + LPS (0 μg / mL) (Control group), (2) EGF (100 ng / mL) + LPS (0 μg / mL) (EGF group), (3) EGF (0 ng / mL) + LPS (1 μg / mL) (LPS group), and (4) EGF (100 ng / mL) + LPS (1 μg / mL) (EGF+LPS group) for 24 h for ussing chamber assay. The transepithelial electrical resistance (TEER, Ω.cm2), glutamine and glucose absorption rate (%) were measured. The results showed that: (1) EGF significantly (P < 0.05) increased the TEER of TPEC-J2 cells induced by LPS; (2) EGF significantly (P < 0.05) increased the absorption of glucose and glutamine of IPEC-J2 cells induced by LPS. It can be speculate that EGF repaired the injured cells by reducing cell permeability and increasing the absorption of glutamine and glucose.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

June

Vol. 53, Iss. 3, Pages 801-1200

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe