Effects of Epidermal Growth Factor on Glutamine and Glucose Absorption by IPEC-J2 Cells Challenged by Lipopolysaccharide using the Ussing Chamber System
Effects of Epidermal Growth Factor on Glutamine and Glucose Absorption by IPEC-J2 Cells Challenged by Lipopolysaccharide using the Ussing Chamber System
Xiaopeng Tang1,2* and Kangning Xiong1,2
ABSTRACT
The aim of this study was to investigate the effects of epidermal growth factor (EGF) on glutamine and glucose absorption of IPEC-J2 cells using the Ussing chamber system. Cells were treated with: (1) EGF (0 ng / mL) + LPS (0 μg / mL) (Control group), (2) EGF (100 ng / mL) + LPS (0 μg / mL) (EGF group), (3) EGF (0 ng / mL) + LPS (1 μg / mL) (LPS group), and (4) EGF (100 ng / mL) + LPS (1 μg / mL) (EGF+LPS group) for 24 h for ussing chamber assay. The transepithelial electrical resistance (TEER, Ω.cm2), glutamine and glucose absorption rate (%) were measured. The results showed that: (1) EGF significantly (P < 0.05) increased the TEER of TPEC-J2 cells induced by LPS; (2) EGF significantly (P < 0.05) increased the absorption of glucose and glutamine of IPEC-J2 cells induced by LPS. It can be speculate that EGF repaired the injured cells by reducing cell permeability and increasing the absorption of glutamine and glucose.
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April 2021
Vol. 53, Iss. 2, Pages 401-800
