In-Vitro Antimicrobial Analysis of Aqueous Methanolic Extracts and Crude Saponins Isolated from Leaves and Roots of Sarcococca Saligna
In-Vitro Antimicrobial Analysis of Aqueous Methanolic Extracts and Crude Saponins Isolated from Leaves and Roots of Sarcococca Saligna
Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*
ABSTRACT
In order to overcome antibiotic drugs resistance, plants provide source for discovery of new antimicrobial drugs. This study was carried out, include in-vitro antibacterial and antifungal screening of aqueous methanolic extracts as well as crude saponins isolated from leaves and roots of Sarcococca saligna, using disc diffusion method. The tested bacterial strains include, B. subtilis, E. coli, S. aureus, P. fluorescens and fungal strains were Aspergillus. niger, A. flavus, and D. turcica. The maximum zones of inhibition, 23.00±0.56 mm and 25.00±0.50 mm was given by ethyl acetate fraction of leaves against P. fluorescens and A. niger respectively. The crude saponins isolated from leaves did not give significant results against the tested bacterial strains where as it was significantly active against fungal strains. The results were compared to standard drug, Ciprofloxacin and Fluconazole which gave 30.0±0.0 mm and 28.0±0.0 mm zone of inhibition respectively. The phytochemical analysis of leaves and roots extracts revealed the presence of alkaloids, saponins, cardiac glycosides, tannins, phenols and flavonoids etc. It is concluded that S. saligna crude extracts as well as crude saponins exhibit broad antimicrobial spectrum against various disease-causing microbes.
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