Submit or Track your Manuscript LOG-IN

Evaluating the Diagnostic Accuracy of GeneXpert MTB/RIF Assay for the Diagnosis of Tuberculosis

Evaluating the Diagnostic Accuracy of GeneXpert MTB/RIF Assay for the Diagnosis of Tuberculosis

Asad Ullah1, Hayat Ullah2, Muhammad Lateef3, Imrana Niaz Sultan4, Afrasiab Khan Tareen4 and Muhammad Waseem Khan4,5*

1Department of Microbiology, Faculty of Life Sciences and Informatics, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, Pakistan.
2Department of Bioinformatics and Biotechnology, Faculty of Life Science, Government College University Faisalabad, Pakistan.
3Biotechnology Department of Centre for Agricultural Biochemistry and Biotechnology Faculty of Agriculture, University of Agriculture, Faisalabad.
4Department of Biotechnology, Faculty of Life Sciences and Informatics, Balochistan University of Information Technology Engineering and Management Sciences, Quetta, Pakistan.
5Department of Environmental and Biological Sciences, University of Eastern Finland, 70200, Kuopio, Finland.
 
* Corresponding author: waseem.khan@uef.fi

ABSTRACT

Tuberculosis (TB) is one of the major global public health concerns particularly affecting population of low-income countries. Early detection of disease coupled with other parameters help in treatment and reducing disease transmission. The current study was conducted to assess the sensitivity and specificity of the GeneXpert MTB/RIF (Cepheid Sunnyvale, CA, United States) in comparison to conventional techniques used for the diagnosis of TB. Our study is one of the first ones from Pakistan investigating and assessing the performance of GeneXpert. We recruited eight hundred clinically TB suspects initially and included seven hundred and sixteen clinically TB suspects in the final analysis. The results of GeneXpert were compared with Mycobacteria Growth Indicator Tube (MGIT) and Ziehl-Neelsen (ZN) staining. In comparison to MGIT and ZN staining the sensitivity of GeneXpert with 95 % confidence interval (CI) was (99.7 %, CI 0.98-0.99) and (95.1 %, CI 0.92-0.97) respectively. The positive and negative predictive values with 95 % CI were (97.1 %, CI 0.94-0.98) and (99.7 %, CI 0.98-0.99) when results of GeneXpert were compare with MGIT results. The results of this study confirm the performance of GeneXpert. With high sensitivity and rapid detection, GeneXpert is ready to be considered as preferred diagnostic tool for TB.

To share on other social networks, click on any share button. What are these?

Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe