Molecular Study of Infectious Canine Hepatitis in Stray Dogs
Molecular Study of Infectious Canine Hepatitis in Stray Dogs
Aiman Mohammed Baqir Al-Dhalimy*, Alaa Kamil Mahmood
ABSTRACT
Infectious Canine Hepatitis (ICH) is a viral disease that is considered a highly contagious and is life-threatening disease in dogs. Canine adenovirus type 1 (CAV-1) is a cause of ICH, which is caused infectious canine hepatitis and uveitis in canine.The study was conducted on 200 stray dogs from three provinces in Iraq (Baghdad, Najaf, Karbala, and Diwaniya). Clinical examinations and signs were documented. Blood samples were obtained from the cephalic vein of dogs with EDTA tubes for molecular assay, and without anticoagulant tubes for serological tests. Polymerase Chain Reaction (PCR) and Enzyme-linked immunosorbent assay (ELISA) tests were used to identify CAV-1. Specific primers targeting the E3 gene were employed for the diagnosis of canine adenovirus type 1 (CAV-1). The PCR results indicated the presence of canine adenovirus type 1 (CAV-1) in 9 out of the 200 animals tested, with the remaining 191 animals testing negative for the virus (4.5%) while the infection rate was five out of 200 stray dogs (2.5%) fixed by ELISA in the survey study. The partial sequences of the E3 gene of three isolates of CAV-1. These sequences of CAV-1 were submitted to the gene bank under accession numbers (PP900637.1, PP900638.1, and PP900639.1), the phylogenetic analysis of CAV-1 Iraqi isolates close to other isolates of isolates as India, Australia, U.K., Canada, Portugal, Italy. The results of the clinical study showed that the Mean of temperature. respiratory rates and pulse rates were significantly increased in the infected dogs with CAV-1. The clinical signs in the infected dogs with CAV-1 included loss of appetite, emaciation, respiratory signs, vomiting, pallor of mucous membranes, depression, and eye cloudiness. This study confirmed some of the clinical manifestations of CAV-1 infection and is considered the first molecular evidence of CAV-1 in Iraq.
Keywords | Molecular study, Adenovirus, Infectious canine hepatitis, Dogs, PCR
To share on other social networks, click on any share button. What are these?
November 2024
Vol. 12, Sp. Iss. 1
