Detection of Intestinal Fungi in Chickens Naturally Infected with Infectious Bursal Disease
Detection of Intestinal Fungi in Chickens Naturally Infected with Infectious Bursal Disease
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Hoda M. Mekky1, Kh. M. Elbayoumi1, 3, Mohamed M. Amer2
ABSTRACT
A little is known about intestinal fungal population and its role during health and disease particularly in chickens. Thus, this preliminary study was performed to identify intestinal fungal population in immunosuppressed chickens. Cloacal swabs were collected from freshly dead chickens suffering from infectious bursal disease (IBD) for isolation of fungal species on specific medium. A total of 19 purified fungal isolates have been identified morphologically. Aspergillus isolates were the most identified (42%), followed by Trichosporon (10.5%), Penicillium (10.5%), Fusarium (5%), Candida (1%) and non-identified isolates (26%). Furthermore, the obtained 8 Aspergillus isolates were further morphologically identified into A. fumigatus which was most frequent species identified (4/19 of total) and (4/8 of Aspergillus species); A. flavus (2), A. niger (1) and A. terreus (1). Molecular identification of 5 representative Aspergillus isolates indicated A. fumigatus (2), A. flavus (2), and A. niger (1) were also performed. We recommended further studies to identify the intestinal fungi of chickens in large scale and different ages which help to understand their role.
Keywords | Aspergillus, Chickens, fungi, immunosuppression, molecular identification, Histopathology.
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