In vitro Evaluation of Mangrove Leaves (Rhizophora apiculata) and Native Grass Based on Phytochemical, Nutritional and Fiber Degradability, Rumen Liquid Characteristics and Gas Production
In vitro Evaluation of Mangrove Leaves (Rhizophora apiculata) and Native Grass Based on Phytochemical, Nutritional and Fiber Degradability, Rumen Liquid Characteristics and Gas Production
Rani Winardi Wulan Sari1, Novirman Jamarun2*, Suyitman3, Khasrad4, Elihasridas2, James Hellyward5, Gusri Yanti1
ABSTRACT
This study aimed to evaluate the combination of mangrove leaves (Rhizophora apiculata) and native grass based on phytochemical contents, nutrient and cells fiber digestibility, rumen fluid characteristic, and gas production using an in vitro methodology. Samples of Hay Mangrove Leaves (HML) (Rhizophora apiculaca) and Native Grass (NG) were arranged into dietary tretmeants (Dry Matter (DM) Basic). The experimental design used is a randomized block design consisting of 4 treatments with 5 replications. The treatment was P1=60% Hay Mangrove Leaves (HML)+40% Native Grass (NG), P2=50% HML+50% NG, P3=40% HML+60% NG, and P4=30% HML+ 70% NG. Data were analyzed using General Linear Model and Duncan’s Multiple Range Test to reveal the significant differences between different treatments applied. The result showed that different ratios between HML and NG contributed significantly differences (p<0.05) in phytochemical contents, in vitro nutrient and fiber fraction digestibility, Volatile Fatty Acid (VFA), Ammonia (NH3), and gas production. No significantly differences (p>0.05) were observed in the pH among the treatments. The nutritional degradation, fiber degradation, VFA and NH3 in the P3 higher than other treatments (p<0.05). P3 had digestibility of dry matter, organic matter, crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF), cellulose, and hemicellulose of 62.82%;65.69%;66.17%;50.67%; 57.22%; 55.84%; 63.27%. VFA and NH3 is 156.20%;9.84%. The methane gas production in P3 was lowest 20.57 ml/gr DM. This research concludes that P3 (40% hay mangrove leaves + 60% native grass) had the best resulted effect on nutrient and fiber in vitro digestibility, rumen fluid characteristic, and gas production.
Keywords | Hay mangrove leaves, Rhizophora apiculata, Native grass, In vitro, Digestibility pathway.
To share on other social networks, click on any share button. What are these?
