Incidence of Fig Leaf Mottle-Associated Virus and fig Mosaic Virus in Eastern Province of Saudi Arabia
Incidence of Fig Leaf Mottle-Associated Virus and fig Mosaic Virus in Eastern Province of Saudi Arabia
Alhudaib, Khalid
ABSTRACT
Fig plant, Ficus carica L., is grown in Saudi Arabia and is being affected by fig plant mosaic diseases (Fig leaf mottle-associated virus, Fig mosaic virus). The main symptoms are chlorotic mottling, blotching and various types of leaf deformation. Samples were collected, with consideration of the economically importance and distribution of the cultivars, from different areas of Hofuf Saudi Arabia. Each sample was consisted of 10-15 leaves. Samples were labelled and stored in plastic bags at 40C; then transferred to the laboratory, for total nucleic acids (TNAs) extraction. One hundred mg of leaf veins and or cortical scrapings were used for extraction. Samples were macerated in 1 ml of grinding buffer. TNAs were recovered with a silica-capture procedure and stored at —200C till used. 8-10gl of TNA extracts were mixed with Igl random hexamer primer, (Boehringer Mannheim, GbmH) (0.5 g/gl). RT-PCR assay of leaves extracts of infected fig accession using specific primers gave positive results and non with FLMaV-2. Mixed infection of FLMaV-1 and FMV were found. To our knowledge this is the first record and identification of FLMaV-1 and FMV in Saudi Arabia. Further studies are needed to investigate the fig mosaic disease throughout the country.
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