Identification and Elimination Treatment of Prunus necrotic Ring Spot Virus (PNRSV) in Rose
Identification and Elimination Treatment of Prunus necrotic Ring Spot Virus (PNRSV) in Rose
* Amal Abou El-Ela, A.
ABSTRACT
Prunus necrotic ring spot Ilarvirus (PNRSV), was isolated from rose shrubs during the survey of rose plantations in Orman Garden, showing Ilarvirus-like symptoms. To identify the causal virus, the plants were tested by enzyme-linked immunosorbent assay using antibodies against different Ilarviruses i.e., Apple mosaic virus (ApMV), Prunus necrotic ring virus (PNRSV), Rose mosaic virus (RNIV) and Tobacco streak virus (TSV) Preliminary results revealed the presence of PNRSV in tested rose shrubs. The isolated virus was biologically purified from single local lesions formed on cucumber cotyledon. Identification of this virus was based on host range, properties in crude sap, transmissibility, and serological tests. PNRSV was able to infect only 12 plant species and varieties from 19 by grafting or mechanical inoculation, it has a limited range of experimental hosts. The dilution end-point of infectivity was 10-3, the thermal inactivation point was 540C, and longevity in vitro was of 10-14 h at 250C. PNRSV was detected by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) was used to amplify a 450 bp cDNA fragment from infected rose leaves (Rosa indica L.) using the primers Macl and Mac2 specific to Prunus necrotic ring spot virus (PNRSV) which were designed to amplify of the 3' end of the coat protein gene (RNA-3)- Nucleic acid hybridization was useful for the detection of PNRSV in herbaceous and woody plant tissues. In successful attempt to eliminate the virus from infected dormant rose cuttings by heat therapy resulted in 29.6% virus elemination of (PNRSV).
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July 2006
Vol. 3, Iss. 1
