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Prevalence and Genotypic Analysis of Hepatitis C Virus Isolated from Liver Center, Faisalabad, Pakistan

Prevalence and Genotypic Analysis of Hepatitis C Virus Isolated from Liver Center, Faisalabad, Pakistan

Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2

1Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad
2Department of Microbiology, Government College University, Faisalabad, 
3Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad 
4School of Biological Sciences, University of the Punjab, Lahore

*      Corresponding author: drmshafique@gcuf.edu.pk; shafique.nl@gmail.com

 

ABSTRACT

Hepatitis C is a serious concern to public health sector, particularly in developing countries like Pakistan. HCV is the significant cause of morbidity and mortality globally due to the lack of HCV vaccine. HCV genotypes are helpful for analyzing disease severity and start of therapy. Therefore, this study was designed to determine the genotyping of clinically isolated HCV from a Liver Center Faisalabad, Pakistan. A total of 3200 clinical samples were screened for the presence of anti-HCV antibodies by ICT method. Among positive samples, confirmation was carried out by RT-PCR and clustered into four groups (acute, chronic, cirrhotic and hepatocellular carcinoma) on the basis of clinical reports and the patient’s history. HCV genotypes were determined by RFLP using amplified products and modified multiplex RT-PCR. Out of 3200 clinical samples, 5.37% (n=172) samples were HCV positive by ICT method. Among these, 96 (55.8%) were confirmed for HCV positive patients. Multiplex RT-PCR data showed that 75 (78%) patients had single HCV genotype infection (3a); three with mixed HCV infection (3a/2a, 3a/3b and 3a/1a) while 18 samples had no HCV signal. The current study demonstrated the high prevalence of HCV in our locality with the most prevalent HCV genotype 3a.
 

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Pakistan Journal of Zoology

December

Vol. 51, Iss. 6, Pages 1999-2399

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