Isolation of Bovine Viral Diarrhea Virus from Calves using Rabbits and Comparative Detection of both Viral Antigen and Antibody for BVDV Genotypes I & II in Experimentally Inoculated Rabbits
Isolation of Bovine Viral Diarrhea Virus from Calves using Rabbits and Comparative Detection of both Viral Antigen and Antibody for BVDV Genotypes I & II in Experimentally Inoculated Rabbits
Ahmed Abd El Samie Hassan Ali
ABSTRACT
Bovine viral diarrhea virus (BVDV) is a world wide pathogen infects a wide variety of ruminants. A random 42 field pooled calf samples were collected and inoculated simultaneously intranasal, oral and intraperitoneal into 6 New Zeeland rabbits. The rabbits were observed for 25 day, slaughtered then tissue harvests from spleen, lung, lymph nodes and intestine were pooled, homogenized; the supernatant was clarified and concentrated for second inoculation. A another 16 BVDV seronegative New Zeeland rabbits, classified into 4 groups, each of 4 designated for second closed inoculation with clarified supernatant of rabbit tissue harvests of first inoculation in comparison to reference BVDV type-I-NADL, BVDV type-2-890 and control negative. Clinical scores and samples included nasal & rectal swabs and sera from rabbits were collected on days, 3, 5, 7, 10, 15, and 21 post inoculations for isolation and detection of its antigens on MDBK cells using outgrowth ELISA and immunofluorescence as well as measurement of BVDV antibodies by serum neutralization test. Virus antigen detection was reported on days 5 & 7 while the antibodies were initially detected on day.
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