Isolation and Molecular Identification of Enterotoxigenic Strains of Escherichia coli in Raw and Pasteurized Milk
Isolation and Molecular Identification of Enterotoxigenic Strains of Escherichia coli in Raw and Pasteurized Milk
Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*
ABSTRACT
As milk is a staple food in human diet consequently the microbiological quality of milk is very important. Therefore, a study was carried out for the molecular characterization (identification) ETEC (Enterotoxigenic Escherichia coli) strains in both raw and pasteurized milk available in the market of District Kasur, Punjab (31.0896° N, 74.1240° E). A total of 65 samples of milk including 5 pasteurized milk samples from various sources were analyzed through scientific polymerase chain reaction (PCR) method including other microbiological laboratory techniques (Total plate count, Gram staining, Hydrogen sulphide, Citrate, Urease and Indole Tests). A high level of total plate count (TPC) and E. coli was observed from both raw and pasteurized milk. The results also indicated a high incidence of ETEC i.e. 63.63% and 50% in raw and pasteurized milk, respectively. The most occurring enterotoxins are ST-I and ST-II (40%) followed by LT-II and ST-II, 20% each, followed by ST-I (13.33%) and LT-I, LT-II (6.66%).
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