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The Effect of Black Cumin Nigella sativa on the Caspase-3 Expression and the Number of Testic Sertoli Cells in Balb/C Male Mouses Exposed to Diazinon

The Effect of Black Cumin Nigella sativa on the Caspase-3 Expression and the Number of Testic Sertoli Cells in Balb/C Male Mouses Exposed to Diazinon

Galuh Chandra Agustina1*, Erwin Syahfitri1, Viski Fitri Hendrawan1, Siska Aditya1,2 

1Faculty of Veterinary Medicine, Brawijaya University,Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151; 2Research Group of Feed and Food Safety, Research Center for Food Technology Processing, The National Agency for Research and Innovation of The Republic of Indonesia, Jl. Jogja-Wonosari, Yogyakarta, Indonesia.

*Correspondence | Galuh Chandra Agustina, Faculty of Veterinary Medicine, Brawijaya University,Puncak Dieng Eksklusif, Kalisongo, Kec. Dau, Kab. Malang 65151; Email: galuh.agustina@ub.ac.id 

ABSTRACT

Diazinon is an organophosphate pesticide that is often used in agricultural business sectors. Nevertheless, this type of pesticide could negatively inhibit the acetylcholinesterase, resulting cell apoptosis and hormonal disruption of the reproductive system. The major of this study was to evaluate the effectiveness of black cumin seeds as antioxidants on the expression of caspase-3 and sertoli cells in spermatogenesis. A total of 20 male mouses were divided into five groups, K- (negative control, no treatment), K+ (positive control, 40 mg diazinon/kg BW); P1 (40 mg diazinon/kg BW + 200 mg black cumin/kg BW); P2 (40 mg diazinon/kg BW + 400 mg black cumin/kg BW); P3 (40 mg diazinon/kg BW + 600 mg black cumin/kg BW) throughout trials. The results showed that the black cumin seeds powder significantly decreased the caspase-3 expression, whereby P3 revealed the lower value compared to other treatment groups (P < 0.05). Besides, an increased the number of Sertoli cells was significantly in line the supplementation of black cumin powder (P < 0.05), showing P3 had stronger effect than their counterpars. Furthermore, this study suggests that the administration of black cumin seed powder could decrease the expression of caspase-3 and increase the number of Sertoli cells in mouses subjected diazinon. 

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Advances in Animal and Veterinary Sciences

July

Vol. 12, Iss. 7, pp. 1206-1409

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