Effect of N-Acetylcysteine Oral Administration on Cutaneous Wound Healing
Effect of N-Acetylcysteine Oral Administration on Cutaneous Wound Healing
Nida Sadaqat1, Sheheyar Ahmed Khan1, Amna Bibi1, Sadaf Zahra1, Muhammad faisal Salamt1, Noreen Latief2 and Fatima Ali1*
ABSTRACT
Thermal injury is prevalent and a burdensome critical care problem due to inadequate safety measures. A severe burn is associated with release of inflammatory mediators including reactive oxygen and nitrogen species (ROS, RNS), which are implicated in pathophysiological events in burn patients. Excessive productions of free radicals are harmful and implicated in tissue damage and multiple organ failure. Supplementation of antioxidants has proven beneficial in decreasing free radicals in burns. The aim of the present study was to investigate the effect of N-acetyl cysteine (NAC) on biochemical alterations during the post-burn stage in scalded rats. Twenty-four male Sprague-Dawley rats were anesthetized and dorsum was shaved. Second-degree skin burn was induced by immersing an iron mold (1.0 cm2 in diameter) in boiling (100°C) water for 5 min and placed on the back of the rats for 20 sec without applying pressure. Rats were divided into three groups; control group, burn injury group, burn injury + NAC group. Wound size and histopathological changes of epidermis were evaluated for the various groups. Hydroxyproline was estimated in wound tissue. Wounded skin tissues were cut off and stored at − 20°C for PCR and ELISA analysis. The therapeutic effects of a NAC supplementation for a short period of time on rat skin wound healing were investigated. The NAC treatment promoted an improvement of wound healing by reducing the oxidative stress, improving wound closure, reducing tissue inflammation with no significant change in blood parameters. There were no significant differences in weekly and final body weights and no significant changes observed in hematological and biochemical markers. A significant increase in collagen synthesis was observed in the NAC treated group with up-regulation of VEGF, IGF-1, PCNA, SDF-1 and CXCR-4 and down regulation of caspase3, P53 and CRP. The study showed that NAC is totally safe to use on rats. Hence for the future it is suggested that more experiments should be conducted for use in humans in conjugation medication for healing burn wounds.
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