Development of a new method for the validation of levofloxacin by UV-Spectrophotometer
Development of a new method for the validation of levofloxacin by UV-Spectrophotometer
MUHAMMAD ASLAM*, ASAD GULZAR, AMINA ASGHAR, MISBAH IRSHAD, ZAHRA NOREEN, SADAF JAVAID, ABRAR HUSSAIN, UMAIR AHMAD & SAMREEN KOUSAR
ABSTRACT
The purpose of the study is to develop an efficient, fast, accurate and
precise UV spectrophotometric method by using 0.1N HCl as a
solvent, for the validation of levofloxacin in injection form. The λmax for
levofloxacin was found to be 293nm using 0.1N HCl as a blank.
Calibration curve data proved that the proposed method was linear in
the concentration range of 3 to 7μg/mL. The proposed method was
validated to determine linearity, precision, accuracy, ruggedness and
robustness. The results of above analytical parameters proved that the
developed method could be used for the routine analysis of
levofloxacin in injection dosage form. The proposed method is free
from expensive solvents, chemicals and time consuming steps and
can be used in quality assurance laboratories of pharmaceutical
industries.
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December 2020
Vol.66, Iss. 2, Pages 125-208
