Biological and Molecular Identification of Severe Zucchini Yellow Mosaic Virus from Cucurbita pepo cv. Eskandarani
Biological and Molecular Identification of Severe Zucchini Yellow Mosaic Virus from Cucurbita pepo cv. Eskandarani
Amel, S.M. Abo-Senna1; M.A. Nasr-Eldin2; B.A. Othman3 and A.A. Megahed4
ABSTRACT
Zucchini yellow mosaic virus (ZYMV) was isolated from Cucurbita pepo cv. Eskandarani cultivated in the open field showing severe systemic symptoms. The isolated virus has wide host range belonging to 4 families resulting systemic and local symptoms. Stability of the isolated virus in vitro was found as 55 oC for TIP, 10-4 for DEP and LIV 4 days. The characteristic spectrophotometric data of the highly purified virus showing that maximum and minimum absorbance was 250 and 260 nm respectively; the ratio for A280/A260 was 1.004; and the Amax./Amin. was 1.099 with the final yield as 0.883 mg/ml. Electron microscopy showed the filamentous flexuous particles with about 750 X 12-15 nm. The virus was identified as ZYMV by RT-PCR technique using high specific primers as 113 bp fragment within the nuclear inclusion protein b (NIb) coding region of the virus genome. The cytopathological effects of ZYMV on the infected cells were completely chloroplasts destruction, and deformation of vascular bundles. The effect of late ZYMV infection decreased fresh weight and yield of squash plants.
To share on other social networks, click on any share button. What are these?
