Protection of Myrmecodia Extract in Tris Diluent on PO Bull Sperm Quality During Freezing
Protection of Myrmecodia Extract in Tris Diluent on PO Bull Sperm Quality During Freezing
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
ABSTRACT
The cooling process causes oxidative stress and affects sperm motility. Antioxidants in the diluent can maintain the motility of bull sperm during the cooling process until after thawing. This research examines the effect of Myrmecodia extract (MYE) supplementation, which has high antioxidants, on semen diluent during the cooling to cryopreservation process. Specifically, five PO bulls aged four years were collected using an artificial vagina (AV) twice weekly at the Semangga District Animal Health Center from August to September 2023. Semen diluted to a minimum of 25 million/mL is packaged in 0.25 mL straws. Treat Andromed® as a comparison, P0 tris egg yolk (TYE), P1 TYE+MYE 1 mL, P2 TYE+MYE 2 mL. The parameters observed include fresh semen quality, sperm motility, sperm viability, sperm membrane integrity, and recovery rate. The results showed that MYE 2 mL supplementation (P<0.05) affected the quality of sperm motility during equilibration and post-thawing compared to P0 and P1. MYE 2mL supplementation in tris diluent has almost the same capabilities as Andromed ® commercial diluent. The ability to maintain cell membrane integrity is indicated by the RR value reaching 77.99%. In conclusion, Supplementation of myrmecodia extract in 2 mL of egg yolk Tris diluent affected sperm protection during the equilibration and cryopreservation processes, following Indonesian National Standard 4869-1:2021 frozen bovine semen.
Keywords | Cryopreservation, Myrmecodia extract, Tris egg yolk, PO bull, Tannin, Protection
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May 2024
Vol. 12, Iss. 5, pp. 802-993
