Microsatellite DNA Markers Revealed Low to Moderate Level of Genetic Diversity in Domestic Stocks of Catla catla
Microsatellite DNA Markers Revealed Low to Moderate Level of Genetic Diversity in Domestic Stocks of Catla catla
Tanveer Ahmed1,2*, Khalid Abbas1*, Huma Naz3, Sajid Abdullah1,
Syed Qaswar Ali Shah3, Adnan Khalil4, Muhammad Sarfraz Ahmed1,
Hina Amjad1 and Shahbaz Ahmad1
ABSTRACT
A total of 15 microsatellite loci in five hatchery populations of Catla catla were examined for the analysis of genetic diversity and genetic structure patterns. For genomic DNA isolation, dorsal muscle tissues of the sampled fish were used. In terms of the average number of alleles and observed heterozygosity, low to moderate levels of genetic variation were found in all the hatchery stocks. The total number of alleles was found to range from 2.800 to 4.000. In all the populations analyzed, FIS values were found to be positive on an average basis, although some loci had negative values. No HWE deviation was found. The pairwise estimates of FST showed low genetic differentiation between populations. Among the individuals in the populations, much of the variance was found by applying AMOVA. Major cluster patterns among all the populations were measured by constructing PCA, structure, and neighborhood joining tree. In the same cluster are those populations that share the most genetic identity, while populations with the lowest genetic identity fall into a separate cluster. In solving the genetic problems associated with restocking plan and brood-stock management strategies of Catla, the present study inferences will be useful.
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