Shedding pattern of classical and variant strain of IB challenge virus in SPF and commercial layer vaccinated chicken with trivalent vaccine containing H9N2 AI, LaSota, classical M41 and var 2 IB viruses
Shedding pattern of classical and variant strain of IB challenge virus in SPF and commercial layer vaccinated chicken with trivalent vaccine containing H9N2 AI, LaSota, classical M41 and var 2 IB viruses
Hanan Sayed 1, Mohamed S. Madkour 1, Hussein A. Hussein 2, Ahmed A El-Sanousi 2
ABSTRACT
Background: Prolonged immune protection for classic and variant IBV in layer chicken rearing is an
important issue.
Objective: Evaluation of the protection duration for IBV (M41 and Var2) post vaccination by
inactivated combined (H9N2, LaSota, M41 and Var2 IB) vaccine based on laboratory tests and
repetition of challenge test.
Methods: We prepared formalin inactivated combined vaccine for Avian Influenza (H9N2N),
Newcastle (La), and IB (M41 and Var 2) by using montanide 71 adjuvant. The prepared vaccine was
injected intramuscularly in specific pathogen free and commercial layer chicken, the injection was
repeated as a booster vaccination after a month from the first vaccination. Humoral immune response
for IBV was measured by ELISA weekly until about one month after the booster vaccination then
periodically till the end of duration. Challenge trial for both IB strain was repeated after a month from
the first vaccination and after two and six months from the booster vaccination.
Results: The use of formalin inactivated combined H9N2, LaSota and IB (M41 and Var 2) vaccine by
using montanide 71 adjuvant via intramuscular vaccination enhanced the immune response as
indicated by ELISA for IB, also induced protection until six months after the booster vaccination
against challenge with IB (Var 2 and M41) viruses.
Conclusion: Usage of formalin inactivated combined H9N2, LaSota and IB (M41 and Var 2) vaccine
with montanide 71 adjuvant can protect chicken until six months from the booster vaccination and
decrease shedding of IBV.
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July 2019
Vol. 6, Pages 1-101
