Uncanny Segmental Molecular Integration of Ahsv and Eev with Btv in Sheep and Goats in Egypt
Uncanny Segmental Molecular Integration of Ahsv and Eev with Btv in Sheep and Goats in Egypt
Mostafa El-Sebelgy1*, Hanafy Madbouly2, Sabry Tamam2, Nagwa Ata1, Kawther Zaher1
ABSTRACT
This study was conducted principally as a trial to expose the molecular identity of the encountered BTV virus and consequently justifying the PCR negativity encountered before. Therefore, the proteomic approach was utilized. The virus was isolated on SPF-ECE and concentrated using PEG-6000. The concentrate was analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) using a technique called untargeted (label-free) shotgun proteomic analysis. The resultant peptides were used along with RefSeq proteins and primers for multiple sequence alignment. Multiple extrinsic proteins [NS1, NS3 and VP3 of African Horse Sickness Virus (AHSV) and NS3 of Equine Encephalosis Virus (EEV)] were detected and at the same time, integration events were examined in this study. This research work entails unpredicted integration of foreign non-BTV viral proteins (NS1, NS3 and VP3 of AHSV and NS3 for EEV). The multiple sequence alignment models elucidated the extent of the integration into the BTV genomic backbone with a resultant insight into PCR negativity. The result confirms the unexpected integration of some segments from equine viruses AHSV and EEV within BTV genome.
Keywords | African Horse Sickness Virus, Equine Encephalosis Virus, shotgun proteomics, Bluetongue Virus, Egypt.
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