Estimation of genetic relationship of wheat germplasm against cereal cyst nematodes using RAPD technique
Estimation of genetic relationship of wheat germplasm against cereal cyst nematodes using RAPD technique
Y. I. Erum and F. Shahina†
ABSTRACT
Investigations were conducted at molecular level, to estimate genetic diversity among wheat germplasm (20 cultivars/lines) in
relation to their response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA
(RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum
percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and
molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the
rest of the genotypes studied and the most effective loci to screen diversity was OPA-09.
To share on other social networks, click on any share button. What are these?
December 2016
Pakistan Journal of Nematology, Vol. 34, Iss. 2, Pages 105-212
