Submit or Track your Manuscript LOG-IN

Infection with the Non cytopathic BVDV-2 Strain 890 Prevents Replication of Superinfecting Cytopathic BVDV-I RNA in BT and MDOK Cells

Infection with the Non cytopathic BVDV-2 Strain 890 Prevents Replication of Superinfecting Cytopathic BVDV-I RNA in BT and MDOK Cells

Ausama A. Yousif1,3, Sandy M. Watkins1, Lyle J. Braunl, David J. Hurley2 and Christopher C. L. Chase1

 

1 Department of Veterinary Science, Animal Disease Research and Diagnostic
Laboratory, South Dakota Stale University, Brookings, SD 5 7007-1396. 
2 Department of Large Animal Medicine. Bldg. 11. University of Georgia. Athens, GA. 30602.
3 Department of Virology, Faculty of Veterinary Medicine. Cairo University, Giza, Egypt.

ABSTRACT

Reports of nonhomologous recombination-events between persistently infecting and vaccinal strains of Bovine virus diarrhea virus (BVDV) indicate that mixed pestivirus infections occur in vivo and that bovine cells support replication of RNA from BVDV-I and BVDV-2 (Ridpath and Bolin. 1995: Becher et al. 2001). BVDV type-specific molecular beacons were used to estimate the effect of the presence of two BVDV RNA genotypes on the RNA relative replication efficiency of each genotype by comparing Ct values obtained from the samne reaction tube in a real-time RT-PCR. This was done in bovine and ovine cultured cells following simultaneous- or superinfection with different biotypes and genotypes of BVDV. The noncytopathic BVDV-2 890 blocked superinfection with the cytopathic BVDV-I strain Singer in both BT and MDOK cells. The cytopathic BVDV strains greatly reduced the relative concentration of superinfecting but not coinfecting cytopathic and noncytopathic BVDV-2 RNA in BT cells. On the Other hand. superinfection of Singer infected cells with BVDV-2 890 increased BVDV-2 890 RNA relative concentration in MDOK cells. 

To share on other social networks, click on any share button. What are these?

Journal of Virological Sciences

July

Vol. 3, Iss. 1

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe