Effects of Epidermal Growth Factor on ASCT2 Expression in IPEC-J2 Cells Challenged by Lipopolysaccharide
Effects of Epidermal Growth Factor on ASCT2 Expression in IPEC-J2 Cells Challenged by Lipopolysaccharide
Xiaopeng Tang1*, Kangning Xiong1 and Rejun Fang2*
ABSTRACT
The aim of this study was to investigate the effect of epidermal growth factor (EGF) on ASCT2 expression in IPEC-J2 cells challenged by lipopolysaccharide (LPS). Cells were treated with: (1) EGF (0 ng / mL) + LPS (0 μg / mL) (Control group), (2) EGF (100 ng / mL) + LPS (0 μg / mL) (EGF group), (3) EGF (0 ng / mL) + LPS (1 μg / mL) (LPS group), and (4) EGF (100 ng / mL) + LPS (1 μg / mL) (EGF+LPS group) for 24 h. The gene expression of glutamine transport protein Na+-dependent neutral amino acid transporter (ASCT2) and protein expression of ASCT2 were measured. The results showed that LPS significantly (P<0.05) decreased the gene and protein expression of ASCT2 in IPEC-J2 cells. EGF significantly (P<0.05) promoted the gene and protein expression of ASCT2. EGF plus LPS group had a higher (P < 0.05) gene and protein expression of ASCT2 than that LPS treated group, although significantly (P < 0.05) lower than EGF treated group, but had no difference between the Control group. It can speculate that EGF promoted the absorption of glutamine through activating ASCT2 expression in injured intestinal epithelial cells.
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