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Bioactive Potential of Leaf Extracts (Tulsi, Curry and Ashoka) Through Total Phenolic Content, Antioxidant, Antimicrobial and Antifungal Analyses

Bioactive Potential of Leaf Extracts (Tulsi, Curry and Ashoka) Through Total Phenolic Content, Antioxidant, Antimicrobial and Antifungal Analyses

Hafiza Mehwish Iqbal1, Salman Khurshid1*, Saqib Arif1, Qurrat-ul-Ain Akbar1, Saba Iqbal2, Shahid Yousaf3, Kainat Qureshi1, Abdul Karim Khan5, Abdul Ahad6, Aqeel Ahmed Siddique4 and Neelofar Hamid7

1Food Quality and Safety Research Institute, SARC, PARC, Karachi, Pakistan; 2Department of Chemistry, University of Karachi, Karachi, Pakistan; 3Food Science Research Institute, NARC, PARC, Islamabad, Pakistan; 4Institute of Plant Introduction, SARC, PARC, Pakistan; 5Mountain Agricultural Research Center, PARC, Gilgit, Pakistan; 6Outreach Research Activities Unit, PARC-SARC, Karachi, Pakistan; 7Department of Botany, University of Karachi, Karachi, Pakistan.

 
*Correspondence | Salman Khurshid, Food Quality and Safety Research Institute, SARC, PARC, Karachi, Pakistan; Email: salmankhurshid67@gmail.com 

ABSTRACT

The current research was carried out for the antimicrobial potential of leaves Tulsi, Curry and Ashoka, targeting two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) as well as two Gram-negative bacteria (Vibrio parahaemolyticus and Escherichia coli) and fungi viz., Aspergillus niger, Alternaria alternate, Fusarium solani and Aspergillus flavus were studied by Well diffusion method. Three solvent extracts Aqueous, Acetone and Methanol with concentrations (5,10,15 and 20%) was prepared. Our study showed that maximum zone of inhibition (ZOI) was observed against bacteria for M. Koenigii i.e., 22mm and 23 mm for fungi, while; O. basilicum and S. asoca plants have21 and 22mm MIZD. Methanol 20%has maximum antioxidant activity in O. basilicum whereas, in S. asoca and M. koenigii 20% aqueous showed maximum activity. Total phenolic content (TPC) 80%methanol exhibited high value in O. basilicum and S. asoca and aqueous 0% has a high TPC content in M. koenigii. The study of medicinal plants is an important area of research in modern medical science for better results and benefits to society and human safety. Use of medicinal plants for antimicrobial and secondary metabolites activities has gained tremendous attention from researchers. 

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Pakistan Journal of Agricultural Research

December

Vol.36, Iss. 4, Pages 297-403

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