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Isolation and Characterization of PI-3 Virus from Sheep and Goats

Isolation and Characterization of PI-3 Virus from Sheep and Goats

JEHAN A. M. GAFER.' HUSSEIN, H.A. and REDA I.M.

* Faculty of Veterinary Medicine, Cairo University, Giza-Egvpt, 12211. •Animal Health Research Institute, Dokki-Giza-Egypt.

ABSTRACT

225 nasal swab samples were collected from diseased sheep and goats for the isolation of PI-3 virus. The samples were taken from diseased animal at seven different Governorates Kaffer el sheikh, Alexandria, EL Behaira, Port Said, Demiatta El-Qalubia and Giza through the winter seasons of years 1999 to 2003. The virus was successfully isolated from three ovine samples after three successive passages on MDBK•cells. The isolated viruses were then titerated and identified using different biological, serological and molecular assay.

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Journal of Virological Sciences

July

Vol. 3, Iss. 1

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