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Seroprevalence of Brucella abortus in Yak, Zo and Cows in Gilgit and Nagar Districts of Gilgit-Baltistan, Pakistan

Seroprevalence of Brucella abortus in Yak, Zo and Cows in Gilgit and Nagar Districts of Gilgit-Baltistan, Pakistan

Waseem Abbas1, Dildar Hussain Kalhoro1*, Hasina Baloch1,  Muhammad Abubakar3, Muhammad Saleem Kalhoro1, Neluma Wali2, Mazhar Hussain Mangi1, Azhar Ali Laghari4, Shahid Hussain Abro1, Rani Wagan1 and Mehkar Hussain5

1Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan
2Department of Zoology, University of Gujrat, Gujrat, Pakistan
3National Veterinary Laboratories, Islamabad, Pakistan
4School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China
5Department of Microbiology, University of Karachi, Karachi, Pakistan
 
*      Corresponding author: [email protected]

ABSTRACT

Brucellosis is one of the most infectious and zoonotic diseases of livestock. Experiments were conducted on serum of yak, zo and cow from Gilgit and Nagar districts of Gilgit Baltistan Pakistan. Seroprevalence and risk factors of brucellosis were recorded during this study. Confirmation of Brucella abortus in serum was carried out using Rose Bengal plate test (RBPT) and indirect enzyme linked immunosorbent assay (I-ELISA). Seroprevalence at district Gilgit was recorded 6.66% in cows and 6.66% in yak while it was 3.33% in zo. Seroprevalence at Nagar district was recorded as 43.33% in cows and13.33% in yak while it was 10% in zo. The overall prevalence at district Gilgit was recorded 5.55% and it was 22.22% at Nagar district. The overall prevalence at summer season recorded was 20% and at winter season it was 7.77%. Highest positive cases were recorded in older animals as compared to young ones. Higher prevalence was observed in female animals as compared to males in both districts. However, all the 180 serum sample examined through RBPT and I- ELISA test suggested that high prevalence of brucellosis in yak, zo and cows in district Nagar as compared to Gilgit and leads to significant economic losses. This is the first study demonstrating the prevalence of an important pathogen in yak, zo and cow of Pakistan.

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Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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